消炎痛对膀胱癌患者抗肿瘤免疫的影响

In Vitro Effects of Indomethacin on Antitumor Immunity in Patients with Bladder Cancer

目的 研究消炎痛 ( IM)对膀胱癌患者 LAK细胞增殖和抗膀胱癌细胞的细胞毒作用的影响。方法 用细胞计数法观察 LAK细胞增殖 ,以膀胱癌组织标本分离的肿瘤细胞和培养膀胱癌细胞系 BIU- 87和 EJ作为靶细胞 ,用 MTT法测定 LAK细胞对肿瘤细胞的细胞毒作用。结果 培养 48至 96小时 IM明显增强白细胞介素 ( IL- 2 )诱导的 LAK细胞增殖 ,IM处理 LAK细胞可增强其对膀胱肿瘤细胞的杀伤。结论 这些研究提示 IM对 IL-

Objective To investigate the combined effects of interleukin 2 (IL 2) with either indomethacin (IM) on the proliferation and cytolysis of bladder tumor cells by lymphokine activated killer(LAK) cells in patients with bladder cancer.Methods LAK cell proliferation was assayed in the presence of various concentrations of IM by cell counting.Bladder cancer cell lines BIU 87,EJ and bladder tumor cells (BTC) from the patients were cultured as target cells,and the cytotoxicity of LAK cells was determined by 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide(MTT) assay.Results The enhanced growth of LAK cells was observed at certain concentrations of IM(100 400 ng/ml)from 48 to 96 h.Pretreatment of LAK cells with IM(200 ng/ml) significantly enhanced cytotoxicity against BIU 87,EJ cells or BTC.Conclusion The inhibition of antitumor immunity can be overcome by IM which may be of use in immunotherapy of bladder cancer.