甲状旁腺激素、1,25(OH)_2D_3对鼠心肌细胞c-myc基因表达的影响

Effects of parathyroid hormone and 1,25-DihydroxyvitaminD_3 on c-myc oncongene expression in cultured rat cardiomyocyte

目的 研究不同浓度水平的甲状旁腺激素 (PTH)和 1,2 5 (OH) 2 D3 对新生大鼠心肌细胞c mycmRNA表达的影响。 方法 采用新生大鼠心肌细胞原代培养和逆转录多聚酶链反应 (RT PCR)技术 ,比较正常对照组、不同浓度PTH组和 1,2 5 (OH) 2 D3 组心肌细胞c mycmRNA表达程度。结果 (1)高浓度PTH组 (10 -8M )心肌细胞c mycmRNA表达较正常对照组和低浓度PTH组(10 -10 M)明显增高 (分别为 1 84± 0 38、0 11± 0 16和 0 10± 0 14,P <0 0 1) ,而后两组之间没有差异 (P >0 0 5 ) ;(2 )两种浓度 1,2 5 (OH) 2 D3 组心肌细胞均未见c mycmRNA表达。 结论 高浓度PTH明显刺激心肌细胞c mycmRNA表达 ,1,2 5 (OH) 2 D3 能抑制c

Objective To study c-myc oncongene expression in cultured rat cardiomyocyte by parathyroid hormone and 1,25(OH) 2D 3.Methods The expression of c-myc oncongene in primary cultured rat cardiomyocyte from control group,PTH group and 1,25(OH) 2D 3 group was detected by reverse transcription-polymerase chain reaction(RT-PCR) analysis.Results (1)The c-myc oncogene expression was significantly increased in high PTH group(10 -8 M) compared with control group and low PTH group(10 -10 M)(1 84±0 38?0 11±0 16 and 0 10±0 14,respectively, P <0 01),There was no difference of the c-myc oncogene expression between control group and low PTH group ( P >0 05).Neither high 1,25(OH) 2D 3 group (10 -8 M) nor low 1,25(OH) 2D 3 group(10 -10 M) showed the c-myc concogene expression.Conclusion The c-myc gene expression is increased by high PTH and is inhibited by 1,25(OH) 2D 3.