二氧化硒诱导的正常肝细胞株及肝癌细胞株的凋亡

SELENIUM DIOXIDE INDUCES APOPTOSIS IN HUMAN NORMAL HEPATIC CELL LINE AND HUMAN HEPATOMA CELL LINE

目的 : 研究中毒剂量二氧化硒 ( Se O2 )对正常肝细胞 HL- 770 2及肝癌细胞 SMMC-772 1的影响。方法 : 用流式细胞术观察不同浓度硒对两种细胞的损伤作用及对两种细胞 Bcl- 2 ,P5 3蛋白表达的影响。结果 : 30 μmol/L的硒作用 48h可以明显抑制正常肝细胞株 HL- 770 2及肝癌细胞株 SMMC- 772 1的增生与活力 ,诱导两种细胞的凋亡 ,且两种细胞伴随着不同程度的 Bcl-2蛋白的下调及 P5 3蛋白的上调。结论 : 中毒剂量的 Se O2 诱导正常肝细胞 HL- 770 2和肝癌细胞 SMMC- 772 1的凋亡 ,且这种诱导凋亡作用是无选择性的 ,但两种细胞调亡的机制有一定区别。

Objective: To investigate the effects of SeO 2 on human normal hepatic cell line HL 7702 and human hepatoma cell line SMMC 7721 and the difference between them. Methods: Two cell lines were treated with 3 to 30 μmol/L SeO 2, and the apoptosis, Bcl 2 and P53 protein expression were detected by flow cytometry. Results: 30 μmol/L SeO 2 markedly inhibited the proliferation and viability, and induced apoptosis more than 50% in both normal hepatic and hepatoma cells after 48 hrs treatment. SeO 2 could also down regulate Bcl 2 protein expression level obviously in HL 7702 but slightly in SMMC 7721, whereas up regulate P53 protein level a little in HL 7002 and significant in SMMC 7721. Conclusion: SeO 2 can induce apoptosis both in human normal hepatic cell line HL 7702 and human hepatoma cell line SMMC 7721, and the mechanism is based on changes of Bcl 2 and P53 protein expression.