枸杞多糖对人白血病细胞株凋亡的影响

EFFECT OF LYCIUM BARBARUM POLYSACCHARIDE ON APOPTOSIS OF HUMAN LEUKEMIA CELLS

目的 : 探讨枸杞多糖 ( LBP- X)对人白血病 HL- 60细胞凋亡的影响。方法 : LBP-X与 HL- 60细胞共培养 ,MTT法检测 LBP- X对 HL- 60细胞增殖的抑制作用 ;以 DPH为荧光探针检测细胞膜流动性的变化 ;用 Hoechst332 5 8染色在荧光显微镜下观察细胞核形态学变化 ;琼脂糖凝胶电泳法和流式细胞仪检测法定性定量检测细胞凋亡。结果 : LBP- X2 0～ 1 0 0 0 mg/L呈剂量依赖性抑制 HL- 60细胞增殖 ,且可降低 HL- 60细胞膜的流动性 ;LBP- X作用 48h后 ,荧光显微镜下细胞核固缩、凝聚和断裂 ,出现浓染致密的颗粒块状荧光 ;DNA琼脂糖凝胶电泳可见明显的“DNA条带”;流式细胞仪分析图上出现凋亡峰。结论 : LBP- X对诱导人白血病 HL- 60细胞凋亡有一定作用。

Objective: To explore the effect of LBP X on apoptosis of human leukemia HL 60 cells. Methods: The inhibitory effect of LBP X on growth of HL 60 cells was assayed by MTT method. The membrance fludity was determined with DPH fluorigenic labeling technique. HL 60 cells were stained with Hoechst 33 258 for nuclear morphology analysis. Agarose gel electrophoresis and flow cytometry were used to analyze apoptosis qualitatively and quantitatively. Results: 20-1 000mg/L LBP X inhibited the growth of HL 60 cells in dose dependent manner and the membrance fludity decreased. The nuclei of HL 60 cells treated with LBP X for 48 h shrinked, condensed and cleaved. Agarose gel electrophoresis of DNA from cells treated with LBP X revealed 'DNA ladder'. HL 60 cells exposed to LBP X showed apoptotic peaks. Conclusion: LBP X can induce apoptosis in human leukemia HL 60 cells.