吲哚美辛胶原蛋白烧伤膜的制备及质量控制

PREPARATION AND QUALITY CONTROL OF THE COLLAGEN BURN PELLICLE OF COMPOUND INDOMETACIN

目的 :研制吲哚美辛胶原蛋白烧伤膜并测定其含量。方法 :以交联剂法制备胶原蛋白烧伤膜 ,并用紫外分光光度法测其含量。结果 :制得乳白色半透明、光滑、具弹性的凝胶膜状物。线性范围为 10 4 4～ 3 6 5 4 μg·ml-1,r=0 9999。方法回收率为 10 0 13 %± 0 0 1% ,日内RSD =0 3 7% ,日间RSD =0 91%。结论 :交联剂法制备胶原蛋白膜简便 ,成膜性好 ;

OBJECTIVE:To prepare the collagen burn pellicle of compound indometacin,we determined its content.METHODS:by using cross linking method,buffer solution was used as solvent,the detective wavelength was set at 265±1 nm?RESULTS:The calibration curve was linear in the range 10.44 36.54 μg·ml -1 , r =0.9999 The average recovery was 100.13% and RSD in a day was 0.37%, RSD between days was 0.91%.CONCLUSION:The method for the quality control of the collagen burn pellicle of compound indometacin is accurate,rapid and simple.