利多卡因对短暂脑缺血后海马区细胞Bcl-2和CPP32蛋白表达的影响

Effect of lidocaine on Bcl-2 and CPP32 protein expressions of hippocampus neurons after transient global cerebral ischemia

目的 探讨短暂脑缺血后 ,利多卡因对海马CA1区细胞两种凋亡相关蛋白表达的影响。方法 通过夹闭家兔双侧颈总动脉和椎动脉 5min ,建立全脑短暂缺血模型。 2 4只家兔随机分三组。对照组 (SH ,n =6 ) :进行手术操作 ,但不夹闭血管 ;缺血组 (IS ,n =9) :夹闭动脉 5min ,夹闭前5min经耳缘静脉予注生理盐水 1 5ml;利多卡因组 (LI,n =9) :夹闭动脉前 5min经耳缘静脉予注2 %利多卡因 10mg kg ,余操作同缺血组。 2 4h后断头取脑。利用蛋白免疫杂交法检测海马区细胞Bcl 2和CPP32蛋白表达。结果 与对照组相比 ,Bcl 2蛋白表达IS组及LI组均明显增高 (P <0 0 5 ) ,LI组高于IS组 (P <0 0 5 ) ;三组之间CPP32表达无明显差异 (P >0 0 5 )。结论 利多卡因可以促进缺血后海马区细胞Bcl 2蛋白的表达 ,进而抑制神经元细胞延迟性死亡。

Objective To evaluate the effect of lidocaine on B cell lymphoma 2 gene (Bcl 2) and CPP32 protein expressions of hippocampus CA1 neurons after transient global cerebral ischemia Both genes are closely related to the development of apoptosis Methods Twenty four healthy adult rabbits weighing (3 0±0 2)kg were randomly divided into three groups: sham operative group (SH group, n=6); ischemia group (IS group, n=9) received normal saline 1 5ml I V 5min before ischemia and lidocaine group (LI group, n=9) received 2% lidocaine 10 ml/kg I V 5 min before ischemia Global cerebral ischemia was induced by the occlusions of bilateral common carotid and vertebral arteries All the rabbits were decapitated 24 h after ischemia and brain tissue was removed immediately Expressions of Bcl 2 and CPP32 proteins of hippocampus CA1 neurons were detected by immunoblotting Results Expression of Bcl 2 in IS group and LI group increased significantly as compared with that in SH group (P<0 05); expressions of Bcl 2 in LI group was obviously higher than that in IS group (P<0 05) There was no significant difference in CPP32 expression between the three groups Conclusions Lidocaine can improve Bcl 2 expression of hippocampus CA1 neurons after transient global cerebral ischemia, but causes no change in CPP32 expression