安氟醚和异氟醚对大鼠中央杏仁核神经元自发放电活动的影响

Effects of enfluane and isoflurane on the spontaneous neural discharge of central amygdaloid nucleus in rats

目的 评价安氟醚 (Enf)和异氟醚 (Iso)对大鼠中央杏仁核 (Ce)神经元自发放电活动的影响。方法 用SD大鼠 ,30～ 6 0d龄 ,雌雄不拘 ,断头 ,迅速取出全脑 ,置于 4℃人工脑脊液 (ACSF)中 ,通入 95 %O2 和 5 %的CO2 混合气 ,制备下丘脑的薄片 ,将 95 %O2 和 5 %的CO2 混合气体经过不同麻醉气体挥发罐 ,安氟醚 (1 5 % ,3 0 % ,4 5 % )和异氟醚 (1 1% ,2 2 % ,3 3% )分别通入ACSF溶液 ,并灌流含Ce脑薄片标本 ,应用膜片钳全细胞记录技术 ,观察给药前、给药期间和冲洗后Ce内神经元自发放电频率的变化。结果 1 5 %～ 4 5 %Enf和 1 1%～ 3 3%Iso具有明显的浓度依赖性抑制Ce神经元的自发放电活动作用 ,3 0 %Enf和 2 2 %Iso对Ce神经元自发放电活动的抑制效应用正常ACSF冲洗 5min可逐渐恢复至给药前水平。结论 Enf和Iso对大鼠神经元自发放电活动可生产明显可逆性的抑制作用 。

Objective To determine the effects of enflurane and isoflurane on the spontaneous neural discharge of central amygdaloid nucleus in rats Methods SD rats (30 60 d) of either sex were decapitated Brain was immediately removed and kept in 4℃ artificial cerebral spinal fluid(ACSF) which was balanced with 95% O 2 and 5% CO 2 gas mixture Braine tissue containing central amyfdaloid nucleus was cut into slices of 300 400μm thick which were immersed in ACSF Enflurane and isoflurane were administered by balancing ACSF with enflurane (1 5%,3 0%,4 5%) or isoflurane (1 1%,2 2%,3 3%) The spontaneous neural discharge of central amygdaloid nucleus was measured before and after enflurane or isoflurane using whole cell patch clamp techniques Results Enflurane and isoflurane inhibited the frequencies of spontaneous neural discharge of central amygdaloid nucleus in a dose dependent manner The spontaneous neural discharge inhibited by enflurane (3 0%) and isoflurane (2 2%) could recover after the slices being washed with normal ACSF for 5 min Conclusions The results indicate that the spontaneous neural discharge of central amygdaloid nucleus can be inhibited reversibly by enflurane and isoflurane Central amygdaloid nucleus may by one of the sites of action of enflurane and isoflurane in central nervous system