上皮性卵巢肿瘤中3p14、3p25杂合性丢失

Loss of heterozygosity at chromosome 3p14 and 3p25 in epithelial ovarian tumor

目的 :经对原发性上皮性卵巢癌的 3号染色体短臂 (3p14和 3p2 5 )杂合性丢失 (LOH)分析研究 ,以探讨 3pLOH与卵巢癌的发生发展之间的相关性。方法 :采用PCR法分别对 5 0例原发性上皮性良恶性卵巢肿瘤 (4 0例癌组织及 10例非癌组织 )的 3p14及 3P2 5处两个特定的位点D3S12 2 8和D3S10 38作杂合性丢失 (LOH)检测。结果 :40例原发性上皮性卵巢癌中在 3p处总LOH率为 80 % ,其中 3p14处发生LOH为 2 4例 (6 0 .0 % ) ,3p2 5处为16例 (4 0 .0 % ) ,两处共同发生LOH的有 8例 (2 0 .0 % )。FIGO卵巢癌的Ⅲa及Ⅲb期患者 3pLOH率分别为93.8%及 91.6 %明显高于Ⅰ—Ⅱ期患者 5 0 .0 %的 3p丢失率 (P <0 .0 5 )。 10例良性卵巢肿瘤患者分别在 3p14和3p2 5各处仅 1例出现杂合性丢失 ,但未见有共丢失现象。 40例不同病理类型的卵巢癌中以浆液性癌 3p14和 3p2 5LOH率为最高 ,但因病例数少而无统计学意义 (P >0 .0 5 )。结论 :3p处可能存在一或多个与卵巢癌发生发展有关的侯选抑癌基因并与卵巢癌的恶性程度相关 。

Purpose:To study the role of ovarian cancer tumor suppressor genes (OCTSGs) and to determine the relationship between 3p loss of heterozygosity (LOH) and the pathogenesis of ovarian cancer. Methods:50 cases of epithelial ovarian tumor, including 40 cases with malignant tumors and 10 cases with benign tumors, were examined by polymerase chain reaction (PCR) with D3S1228 and D3S1038 microsatellite polymorphic markers at 3p. Results:Thirty two of the 40 cases (80%)with ovarian cancer showed either LOH at 3p14 or at 3p25, but only one of 10 cases (10.0%)with bengin ovarian tumor there occured 3p LOH. Among them, 24 cases (60.0%) had LOH at 3p14 and 16 cases (40.0%) at 3p25, as well as 8 cases (20.0%) there was co deletion on both at 3p14 and 3p25. According to FIGO staging, the rate of LOH at 3p in ovarian cancer patients with Ⅲa , Ⅲb was higher than that in patients with stage Ⅰ, Ⅱ ( P <0.05), however, there was no relationship between 3p LOH and the pathological types in epithelial ovarian cancer ( P >0.05). Conclusions:Since 3p14 and 3p25 LOH are related to the staging of ovarian cancer as well as the common occurrence of 3p LOH in epithelial ovarian cancer, it is suggested that there are some candidate ovarian TSGs harbored at 3p regions and the detection of 3p LOHs may be used as a genetic marker for monitoring the development of ovarian cancer.