正常人和原发性开角型青光眼患者小梁网M_3受体的表达

Muscarinic receptor subtype-3 expression in trabecular meshwork of normal human eye and primary open-angle glaucoma

目的 研究正常人和晚期原发性开角型青光眼 (primaryopenangleglaucoma ,POAG)患者小梁网M3 受体的表达 ,探讨POAG患者小梁网的病理生理改变。方法 取 5例正常人及 10例晚期POAG患者小梁网组织标本 ,应用免疫组化方法检测小梁细胞M3 受体 ,并利用计算机图像分析系统对检测结果进行分析。结果 5例正常人的小梁网组织标本有M3 受体表达 ,阳性细胞主要分布在葡萄膜小梁网处 ,前至Schwalbe线 ,后达巩膜突。晚期POAG患者小梁网组织标本中 ,小梁细胞数量明显减少 ,M3 受体阳性细胞亦相应减少且散在分布 ,部分标本甚至无阳性M3 受体表达。结论 正常人小梁网细胞M3 受体表达阳性 ,晚期POAG患者小梁网M3 受体阳性细胞数量明显减少且分布不规则。

Objective To identify the muscarinic receptor subtype-3 (M 3-receptor) expression in trabecular meshworks (TM) of normal human eyes and eyes with primary open-angle glaucoma (POAG) and discuss the pathological changes of TM in POAG. Method TM specimens were taken from 5 normal persons and 10 patients with POAG at late stage. Then LSAB immunohistochemical technique was used to identify M 3-receptors. The results were observed under microscope and compared with computer-image-analysis system. Results (1) The trabecular meshwork cells in all the 5 normal persons expressed M 3-receptors. The positive cells mainly existed in the uveal part of TM, from Schwalbe′s line to scleral spur. (2) In TM from eyes with POAG, the amount of both TM cells and M 3-receptor positive cells were reduced obviously, even some specimens had no such positive expression. The positive cells were correspondingly decreased in number and scattered in distribution. Conclusion The TM cells in normal human eyes express M 3-receptors. The TM cells and M 3-positive cells in eyes with late POAG are reduced in number and their distribution is irregular.