体外诱导绵羊红细胞特异性抗体形成及单纯疱疹病毒感染对它的影响

In Vitro Induction of SRBC-sgecific Antihody by Human Tonsillar Lymphocytes and the Effects of HSV-1 Infection on it

本文建立了一种较稳定、理想的人淋巴细胞体外诱导绵羊红细胞(SRBC)特异性抗体生成的系统。用SRBC体外刺激人扁桃体淋巴细胞,用溶血空斑法计数针对SRBC特异性抗体形成细胞。发现极低量抗原可诱导其抗体形成,抗体形成量随抗原量呈规律性变化;在抗原刺激后的第4天特异性抗体开始出现,第6天达高峰,并稳定维持至第8天;在辅助刺激剂美洲商陆(PWM)存在下,抗体形成量显著高于无PWM的情况;除去人扁桃体细胞中粘附细胞(主要是巨噬细胞)才能诱导最适抗体形成。将具感染性的HSV-1与SRBC一起加入淋巴细胞培养中,可显著抑制SRBC诱导的特异性抗体形成,这一抑制效应与病毒的感染量有关。此系统中同时加入α-干扰素则可部分解除病毒的抑制效应,并且解除效果与α-干扰素的剂量有关。

We established a stable human tonsillar cell culture-immunization systemin vitro, in which sheep red blood cells (SRBC), a virus-irrelevant antigen,induced efficiently specific antibody production. In contrast to traditional con-cepts, we demonstrated that adhering cells (mainly macrophages) in tonsilsplayed a suppressive role in induction of specific antibody response in vitro.Depletion of adhering cells increased significantly the production of this anti-body. We also found the SRBC co-stimulated with suitable dose of PWM couldinduce the highest antibody response. SRBC-induced specific antibody produc-tion reached its peak at the sixth to eighth day. Results suggested that this invitro immunization system be suitable and convenient to be used on studyingeffects of virus infection on specific immune response in virto.Data further showed that the suitable dose of livig HSV-1 added into thesystem decreased dramatically SRBC-induced specific antibody production invitro. The suppressive effects of livig HSY-1 could be eradieated by additionof IFN-a.