乙肝病毒adr型基因疫苗诱导小鼠体液免疫应答

Humoral immune response to gene vaccine against adr hepatitis B virus

目的 :构建乙肝病毒 adr型基因疫苗 p CMVS2 - S,观察其免疫小鼠的特异性体液免疫应答。方法 :将构建的乙肝病毒基因疫苗注射于 C5 7BL/ 6小鼠胫骨前肌内 ,运用 EL ISA检测不同时间小鼠血清中抗 HBs抗体。结果 :注射基因疫苗 1周后 ,血清中抗 HBs抗体转阳 ,1个月后抗体达到高峰 ,并以高水平维持至少 2个月。 结论 :基因疫苗 p CMVS2 - S能诱导 C5 7BL/ 6小鼠产生较好的体液免疫应答。

Objective: To construct HBV adr gene vaccine pCMVS 2-S and to observe the specific humoral immune responses in C57BL/6 mice after gene immunization. Methods: The HBV gene vaccine was injected into tibialis anterior muscles of C57BL/6 mice. ELISA was used to detected the anti-HBs antibody in mice sera at various time points after gene transfer. Results: Anti-HBs in sera of mice was tested positive 1 week after plasmid injected, the level of antibody peaked 4 weeks later, and kept high titer for at least 2 months. Conclusion: The good response of humoral immunity can be induced by injection of pCMVS 2-S in C57BL/6 mice.