神经生长因子对新生大鼠缺氧缺血性脑损伤后神经元特异性烯醇化酶的影响

The effect of nerve growth factor on neuron-specific enolase after hypoxic-ischemic brain damage in neonatal rats

目的 研究神经生长因子 (NGF)对新生大鼠缺氧缺血性脑损伤 (HIBD)的作用。方法 应用新生7日龄SD大鼠制备HIBD模型 ,于缺氧缺血 (HI)后即刻腹腔注射NGF 10 0U/只或等量生理盐水 ,用放射免疫法测定缺氧缺血后 2 4、48h血清神经元特异性烯醇化酶 (NSE)水平 ,用免疫组化法检测脑组织NSE蛋白表达变化。结果 NGF可降低血清NSE ,增加左侧 (缺血侧 )脑组织中NSE蛋白水平表达。结论 腹腔注射NGF对HIBD具有保护作用。

Objective To expore the effect of nerve growth factor(NGF) on hypoxic-ischemic brain damage(HIBD) in neonatal rats. Methods The HIBD models were established with 7-day postnatal SD rats.Right after hypoxic-ischemic insult,NGF 100U per rat or normal saline solution was injected intraperisoneally.At 24 hours and 48 hours after hypoxic-ischemic insult,values of neuron-specific enolase(NSE)in serum were measured by radioimmunoassy and immunohistochemical technique was used to investigate the changes of expression of NSE in cerebral tissues. Results The use of NGF lowered serum NSE and increased the protein expression of NSE in the left cerebral tissues. Conclusion It is suggested that intraperitoneal administration on NGF can protect against hypoxic-ischemic brain damage(HIBD) in neonatal rats.