PLC-γ1对PDGF介导的细胞内钙流的影响

The effect of PLC-γ1on mobilization of intracellular Ca2+ induced by PDGF

磷酸脂酶C-γ1(PLC-γ1)可催化PIP2生成DAG和IP3,后二者被认为是细胞内第二信使。其中IP3可使细胞内钙库的Ca2+释放到胞浆。应用无内源性PLC-γ1小鼠成纤维细胞株,观察在血小板生长因子PDGF作用下细胞内钙流的产生情况,发现缺失PLC-γ1的细胞与正常细胞都出现细胞内钙动员。但前者钙流产生较后者值小且峰值出现较晚。这意味着PLC-γ1在PDGF作用下成纤维细胞内钙流的产生过程中有重要作用,当其缺乏时功能可被其它途径代偿。

Phospholipase-gamma1(PLC-γ1)mediate the hydrolysis of phosphatidylinositol4,5-dipho-sphate(PIP2)to the second messenger molecules inositol1,4,5-triphosphate(IP3)which increases intracellular levels of free Ca 2+ and diaclglycetol(DAG).Using the immortalized fibroblasts genetically deficient in PLC-γ1(PLC-γ1 -/- )to investigate the role of PLC-γ1in mobilization of intra-cellular Ca 2+ induced by platelet-derived growth factor(PDGF),we found in PLC-γ1 -/- cells,the mobilization of Ca 2+ were attenu-ated and postpone.Therefore,it seems that the role of PLC-γ1is important in cells mitogenic signal transduction induced by PDGF.,and may be substituted by other signal molecules.