带有白花甜菜染色体的栽培甜菜单体附加系RAPD分析适宜引物的筛选

Screening of optimal primers of RAPD analysis in monosomic addition lines of B. Vulgaris L. with a chromosome of B. corolliflora Zoss

运用 ６８个 １０ｂｐ随机引物对白花甜菜、载培甜菜、带有白花甜菜染色体的栽培甜菜单体附加系进行了ＰＣＲ扩增，所选引物中有２个扩增产物不明显，４４个引物扩增产物在所选材料中无多态性，２２个引物的扩增产物对白花甜菜具有特异特殊异片段，这 ２２个引物将可以用于白花甜菜单体附加系的ＲＡＰＤ分析研究。

We have performed the RAIPD analysis in B.corolliflora Zoss., B.Vulgaris L., monosomic addition lines of B.corolliflora. Of total tested 68 random primers, the amplification products of two primers are not visible; 44 primers have consistent amplification products in selected materials; 22 amplification products have specific fragment in B. corolliflora. Thes 22 random primers will be applied to RAPD analysis of B. Vulgaris monosomic addition lines.