NOS抑制剂对红藻氨酸诱导癫痫发作大鼠脑海马组织细胞凋亡的影响

Effect of NOS inhibitor on neuronal apoptosis in rat brain during kainic acid-induced seizures

目的 研究红藻氨酸诱导癫痫发作大鼠脑组织中一氧化氮合酶 (NOS)抑制剂与海马组织细胞凋亡的关系。方法 利用流式细胞术 ,测定红藻氨酸诱导大鼠癫痫发作及用L 精氨酸 (L Arg)的硝基衍生物N 硝基 左旋精氨酸 (L NNA)干预后的鼠脑海马组织细胞凋亡情况。结果 在癫痫发作过程中 ,NOS抑制剂对鼠脑海马组织细胞凋亡峰有明显影响 ,L NNA能增加凋亡发生率。结论 在癫痫发作过程中 ,鼠脑海马组织存在细胞凋亡现象 ,NOS抑制剂对凋亡有明显影响。

Objective To study the role of nitric oxide synthase (NOS) inhibitor effecting on neuronal apoptosis in the epileptic seizures induced by kainic acid(KA). Methods Flow cytometry(Fcm) was used to observe changes of neuronal apoptotic rates in epilepsy experimental rats were treated with L NNA via intraperitined injection. Results NOS inhibitor effected the percentage of DNA fragmentation in the epileptic seizures, L NNA can increase incidence of neuronal apoptosis. Conclusion There is cell apoptosis in the brain regions in epilepsy. NOS inhibitor influences on incidence of neuronal apoptosis.