炎症信号受体Toll基因在人肺腺上皮细胞和血管内皮细胞的表达

Human Toll Like Receptor Gene Expression in Human Pulmonary Granule Epithelial Cells and Umbilical Vein Endothelial Cells

目的 研究炎症信号受体 Toll m RNA在人单核吞噬细胞系 (THP- 1)、脐静脉血管内皮细胞(UVECs)和肺腺上皮细胞系 (SPC- A- 1)的表达情况。方法 分别设计人 TL R2 和 TL R4两对特异引物 ,从 THP- 1、UVECs和 SPC- A- 1提取总 RNA,采用一步法 RT- PCR和地高辛标记的 Northern杂交技术检测 TL R2 和 TL R4在 3种细胞的表达。结果 RT- PCR和 Northern杂交显示 ,3种细胞均表达 TL R4受体 ,但仅 THP- 1和人 U VECs表达 TL R2 受体。结论 本实验结果表明除单核吞噬细胞外 ,大血管内皮细胞和肺腺上皮细胞亦表达 Toll受体基因 ,提示在炎症反应中 Toll信号受体可能介导这些细胞效应分子基因转录激活的信号传导。

Objective To determine whether human Toll like receptors (TLRs) genes are expressed in human lung granule epithelial cells and umbilical vein endothelial cells (UVECs). Methods Specific primers for TLR 2 and TLR 4 were designed and total RNAs were isolated from human monocyte cell line THP 1, human lung granule epithelial cell line SPC A 1 and the primary culture of human UVECs. RT PCR and Northern blotting with digoxin labeling were used for the detection of TLR 2 and TLR 4 mRNA expression. Results TLR 4 mRNA expressed in the three types of cells mentioned above. TLR 2 mRNA was detected in THP 1 and UVECs, but not in SPC A 1 cells. By using DNA sequencing, the RT PCR products were confirmed to be TLR 2 and TLR 4 cDNAs. Conclusion This study provided evidence indicating that Toll like receptor genes constitutively express not only in human monocytes but also in human larger vascular endothelial cells and pulmonary granule epithelial cells.