具有电学活性的硬组织替代材料的骨内植入实验研究

An Experimental Study on the Implantation of a Biomaterial with Electro-activity for Replacement of Hard Tissue in Bone

目的 研究生物压电陶瓷植入材料对机体骨组织生成的影响。方法 选择与机体骨组织压电性能相适应的压电陶瓷材料 HABT(hydroxyapatite and barium titanate)和对照材料羟基磷灰石 (hydroxyapatite,HA)植入到狗的双侧下颌牙槽骨中 ,分别在 1周、2周、1个月、2个月、3个月处死动物 ,取材制样 ;另将极化后的HABT垂直植入猴一侧下颌骨 ,另侧设空白对照 ,34个月后取材制样 ,以光镜和扫描电镜对样品进行组织形态学观察。结果 HABT植入狗下颌牙槽骨 1周后即有小片钙化的新骨生成 ,材料 -骨界面新生胶原呈有规律的定向束状排列 ,排列方向与压电材料极化方向垂直。而 HA植入 1周时仅有排列无明显方向性的胶原纤维生成。HABT植入猴下颌骨 2年零 10个月后 ,材料与骨组织融为一体 ,骨结构正常。结论

Objective To assess the effects of a piezoelectric biological ceramic on osteogenesis. Methods Hydroxyapatite (HA) and piezoelectric biological ceramics (hydroxyapatite and barium titanate, HABT) were implanted in the jawbones of 5 dogs, and for sample collection, the dogs were killed separately at 1 week, 2 weeks, 1 month, 2 months and 3 months after implantation. The samples from a rhesus monkey and a blank control were collected 34 months after implantation. The implanted samples and surrounding tissues were subjected to histological observations using light microscopy (LM) and scanning electronmicroscopy (SEM) were made. Results Compared with the control groups, the HABTs promoted osteogenesis significantly. One week after implantation, new bone tissues were found on the surface vertical to the longitudinal direction of HABT; more bone tissues were found after 2 weeks. HABTs induced the bone tissues to arrange orderly. After two years and ten months of implantation, the piezoelectric bioceramic and bone became monolithic, and the structure of bone was normal. Conclusion HABTs could promote osteogenesis.