钙离子选择性微电极在钙制剂药代动力学研究中的应用

Application of Ca^(2+)-ISME in the Studies on Pharmacokinetics of Calcium Supplements

目的 用自制的钙离子选择性微电极 (Ca2 +- ISME)测定游离钙 ,拟反映体内钙被吸收的真实水平。方法 建立了血清样品中钙离子测定方法 ,并进行三种具有代表意义的补钙剂的大鼠药代动力学对比研究。经灌胃给药 ,定时取血 ,制备血清并测定其钙离子浓度。结果 三种制剂在血中的达峰时间不完全一致 ,但其 AU C间经 t检验无显著性差异。结论 Ca2 +-

Objective To determined the concentration of Ca 2+ in serum by a self made Ca 2+ ISME in order to indicate the absorption of calcium supplement. Methods We have developed a calibration curve method to measure Ca 2+ activities in serum sample. The pharmacokinetics of three kinds of calcium supplements were investigated by the Ca 2+ ISME after P.O. administration in 5 healthy rats. Results Though the T max (hours) of the three kinds of preparations were different, there were no significant differences among their AUC by paired t text. Conclusion The above results demonstrate that our self made Ca 2+ ISME is applicable to the researches on pharmacokinetics of calcium supplements.