同源盒基因Hox2.3在小鼠造血调控中的作用

THE COMMITMENT ROLE OF HOMEOBOX GENE IN THE REGULATION OF MURINE HAEMATOPOIESIS

同源盒基因在造血细胞系细胞中表达,它们的表达具有细胞类型特异性。为了阐明同源盒基因在小鼠造血调控过程中是否有决定性作用,我们采用反义脱氧寡聚核苷酸抑制试验和逆转录-聚合酶链式反应(RT-PCR)试验,证明小鼠同源盒基因(Hox2.3)对正常小鼠髓系血细胞的生长、发育调控起重要的作用。

In this study, we found that Hox gene was expressed in the normal murine bone marrow cells after stimulation of colony stimulating factors (CSFs). By using antisense oligodeoxynucleotide inhibition strategy, we showed that the Hox gene expression was specifically inhibited by its antisense oligomers, The treatment of bone marrow cells to Hox gene antisense oligomers also led to the inhibition of CFU-GM formation. These findings indicated that Hox gene plays a critical role in regulating normal murine haematopoiesis.