诊断级超声对人早孕绒毛组织中NO及NOS的影响

Effect of diagnostic ultrasound on NO and NOS in human intra uterine villi in early pregnancy

目的 通过检测孕早期诊断级超声辐照后人绒毛组织中一氧化氮合酶 (nitricoxidesynthase,NOS)活性及一氧化氮(nitricoxide,NO)含量的变化来评价孕期超声诊断的安全性。方法 采用细胞培养技术 ,生化测定技术检测绒毛细胞培液中相关生化指标的变化。结果 ①NOS活性测定 ,实验组明显低于对照组 (P <0 0 1) ,10min组值更低 ;②NO含量测定同样是实验组明显低于对照组 (P <0 0 1) ,10min组与对照相比差异更显著。结论 孕早期接受一定剂量的超声辐射可使胎盘组织细胞中的NO含量下降 ,NOS活性减弱。

Objective By detemining the changes of NOS and NO levels in early pregnancy women exposed to ultrasonic diagnostic dosage to assess the diagnosis safety. Methods Cell culture and biochemical technique were adopted to study the changes of NO and NOS in chorion cultural solution. Results The NOS and NO levels were significantly lower in the treated groups than in control group( P< 0 01),and the lowest level was seen in the 10 min group. Conclusion Diagnostic ultrasound in early pregnancy can make NOS and NO levels decrease.