妊娠高血压综合征患者外周血T_H1和T_H2细胞比例变化的研究

A Study on the Ratio of T_H1, T_H2 Cell in the Peripheral Blood from Patients with Severe Pregnancy Induced Hypertension

目的 研究妊娠高血压综合征 (简称妊高征 )患者 TH1和 TH2型细胞免疫反应的变化 ,从免疫学角度探讨妊高征的发病机理。 方法 利用 Per CP-CD4、FITC-IFN-γ和 PE-IL-4抗体三重染色 ,流式细胞术测定重度妊高征患者和正常晚期妊娠妇女外周血 CD4阳性细胞中分泌细胞因子IFN-γ的 TH1细胞和分泌细胞因子 IL-4的 TH2细胞所占的比例。 结果 ( 1 )重度妊高征组外周血中 TH1细胞比例为 ( 3 8.0 1± 9.0 4 ) % ,明显高于正常晚期妊娠组 ( 3 0 .2 6± 8.65) % ,而 TH2细胞比例( 2 .2 5± 0 .61 ) %显著低于正常晚期妊娠组 ( 5.56± 1 .2 9) % ,P均 <0 .0 5,( 2 )重度妊高征组 TH1 / TH2比值为 1 7.2 1± 9.0 2 ,显著高于正常晚期妊娠组 7.0 4± 5.3 0 ,P<0 .0 1。 结论 重度妊高征患者与正常晚期妊娠妇女比较 ,其体内表现为免疫杀伤的 TH1型免疫反应增强 ,而表现为免疫保护或免疫营养作用的 TH2型免疫反应减弱。提示 :TH1与 TH2平衡失调 ,即当 TH1型免疫反应占优势时则可诱发妊高征。

Objective To investigate the immunologic etiology of pregnancy induced hypertention (PIH)by studying the changes of T H1 and T H2 cell ratio in the peripheral blood from patients with severe PIH. Methods We examined the cell percentage of T H1 cell(secreting cytokine IFN γ) and T H2 cell(secreting cytokine IL 4),which were from CD4 positive, by flow cytometry tri stained with PerCP CD4,FITC IFN γ and PE IL 4 monoclonal antibody. The blood samples were from severe PIH patients and normal third trimester patients. Results The percentage of T H1 cell 38.01±9 04% from the peripheral blood of severe PIH partuients was significantly higher than that of the normal partuients of the third trimester 30.26±8.65%, while the percentage of T H2 cell 2.25± 0.61% was much lower P <0.05, and the ratio of T H1/T H2 was elevated significantly, P <0.01. Conclusions Compared with the normal parturients, the severe PIH patients have the elevated T H1 type immuno reactions which expresses immune damage and weakened T H2 type immuno reactions which is immunoprotection or immuno nutrition. These suggests that the imbalance of T H1 and T H2, especially when the T H1 type immuno reaction is dominant, may induce PIH.