微阵列分析肝癌组织中的整合素

Integrin gene expression profiles in liver cancer with microarray

目的了解肝癌中整合素的表达概况,探寻在肝癌及正常肝组织中的表达差异。方法以肝癌及癌旁正常肝组织的总 RNA 反转录合成含有α^(32)P dATP 的 cDNA 为探针,与 Atlas 微阵列表达分析膜进行差异杂交,并应用半定量 RT-PCR 验证。结果放射自显影结果经 AtlasImage^(TM)软件分析显示:在所分析的588种已知基因中,整合素相关的基因共有17个,其中 integrin alpha8、betal、beta7、beta8等4个在肝癌组织中表达上调。结论通过 Atlas 微阵列系统分析发现的这些基因的表达改变组成了一个肝癌特异的基因表达谱,是人类首次全面系统地研究肝癌的基因表达改变,整合素基因的差异表达为研究肝癌的侵袭、粘连和转移机制提供了有益的线索。

Objective To describe a liver cancer-specific gene expression profile and to identify the difference of the expression between liver cancer tissue and its adjacent tissues.Methods The cD- NA probes labeled with α-^(32)P dATP were synthesized from total RNA of cancerous tissue and its adja- cent tissues and differentially hybridized to 2 identical Atlas human cancer cDNA expression array membranes containing 588 known genes.A gene-specific semiquantitative reverse transcription poly- merase chain reaction(RT-PCR)method was used to confirm the expression pattern of integrin betal. Results Autoradiographic results analyzed by specific Atlaslmage^(TM)(version 1.01a)showed that 17 genes out of the 588 were related to integrin.Four of them,including alpha8,betal,beta7 and beta8 were up-regulated in the cancer.RT-PCR demonstrated that the expression pattern of integrin betal was consistent with the results of Atlas human cancer cDNA expression array.Conclusions The re- sults obtained from Atlas microarray provide a liver cancer-specific expression profile for the first time.This profile can identify the gene expression comprehensively and systematically.In addition, the finding of integrin gene expression may lead to finding clues of the cell adhesion and invasion mech- anism of liver cancer.The differential hybridization technique of AtlasTM human cancer cDNA ex- pression array can be a useful method in understanding the occurrence and progression of human canc- er.