生长抑素基因初级克隆质粒(pUC12-SS)的改造与鉴定

MODIFICATION AND IDENTIFICATION OF THE PRIMARY SOMATOSTATIN GENE CLONED PLASMID

生长抑素(somatostatin,ss)基因初级克隆质粒(pUC12-SS)含有限制性内切酶BamHI和HindⅢ的重复位点,用HindⅢ酶切处理和T_4DNA连接酶连接后。获得了它们的单一位点。并对经过修饰后的质粒进行了多个酶位点、SS基因片段回切和DNA序列等全面分析和证实。

Immunization against somatostatin (SS) has been shown to stimulate the growth of animals with the increase of growth hormone in the plasma. To develop an SS vaccine with high immunogenicity, thc fusion of SS gene with hapatitis B virus antigen (HBsAg or HBcAg) gene is designed since the fusion protein can self-assemble into polymer particles which may present large amount of SS antigcnie determinants on their surfaces. This paper deais with the modification and identification of the primary SS gene cloned plasmid (pUC12-SS). The pUC12-SS with dual sites of BamH Ⅰ and HindⅢ was digested with ItindⅢ. As a result, unique cndonuclcase sites were produced for the gene fusion. Thc cndonuclcase sites related, SS gene fragment and DNA sequence were determined.