盐胁迫对大、小麦种子萌发时两种酶活性的影响

EFFECTS OF SALINITY ON ACTIVITY OF TWO ENZYMES IN BARLEY AND WHEAT DURING EMERGENCE

在200mmol/L NaCl处理下,大、小麦种子内α-淀粉酶活性降低,贮藏物质转化受阻,小麦比大麦更明显。分别以100,200mmol/LNaCl处理幼苗2天,大麦幼根的质膜、液泡膜微囊H^+-ATPasc活性均增大,并且随盐浓度的增加而增强;小麦在100mmol/L NaCl处理下,幼根液泡膜微囊H^+-ATPase活性增强,而在200mmol/L NaCl处理下,质膜、液泡膜微囊H^+-ATPase活性均下降。200mmol/L HaCl处理9天,大、小麦幼根质膜、液泡膜微囊H^+-ATPaso活性都下降,小麦的下降幅度大于大麦,盐胁迫对大、小麦种子萌发和幼苗生长的不同效应,主要与种子内贮藏物质的转化以及根系对离子的选择吸收及运输有关。

Under 200 mmol/L NaCl, the activity of α-amylase in barley and wheat seeds decreased, the transformation of stored substances in seeds was inhibited, and both of them were more obvious in wheat than in barley. Barley and wheat seedlings were treated with 100 and 200 retool/L NaCl for 2 days, the H^+-ATPasc activity of plasmalemma and tonoplast vesicles isolated from barley immature roots increased along with NaCl concentrations. For wheat, the H^+-ATPase activity of tonoplast vesicles increased under 100 mrnol/L NaCl, and the H^+-ATPase activity of plasrnalemma and tonoplast vesicles decreased under 200 mmol/L NaCl. Under 200 mmol/L NaCl for 9 days, the H^+-ATPase activity of plasmalemma and tonoplast vesicles decreased, the extent of decrease was greater in wheat than in barley.