摘要
采用全血涂片免疫酶染法建立了检测T细胞亚群的新方法。该法具有良好的敏感性、特异性、稳定性与重复性。经与传统的间接免疫荧光法及单个核细胞涂片免疫酶染法作对比研究,证明该法具有需血量少、省时、经济和简便等优点。应用该法检测了48例EHF患者及32例慢性HBV感染患者的外周血T细胞亚群。结果显示EHF患者极期CD8^+T细胞百分比明显升高,CD4^+/CD8^+比值下降或倒置。慢性HBV感染患者也显示CD8^+细胞百分比升高,CD4^+/CD8^+比值下降。本文还对EHF和慢性HBV感染患者T细胞亚群变化的意义进行了讨论。
A sensitive, specific and reproducible method was established for identifing T lymphocyte subpopulation by immunoperoxisase staining of whole blood smears. Compared with conventional indirect immunofluorescence method and immunoperoxidase staining of PBMC smears, this method has the following advantages: need not to separate PBMC, smaller blood sample, time-saving and easier to perform. Peripheral blood T-cell subpopulations were detected by this new method in 48 patients with epidemic hemorrhagic fever (EHF) and 32 patients with chronic HBV infection. It was found that in EHF patients CD8^+ T-celi percentage significantly increased and CD4^+ T-cell percentage sightly decreased, resulting in a reversed CD4^+ /CD8^+ ratio. The results of chronic HBV infection patients showed increased CD8^+ T-cell percentage with a decreased CD4^+ /CD8^+ ratio. The significance of T-cell subpopulation variations in the pathogenesis of EHF and chronic HBV infection is discussed.
关键词
免疫酶技术
T细胞亚群
乙型肝炎
immunoenzyme technics
suppressor cells
hemorrhagic fever
epidemic
hepatitis B
