L-乳酸高产突变菌株—米根霉NAF-032菌种培养条件的研究

STUDY ON CULTURE CONDITION FOR L-LACTIC ACID HIGH-PRODUCING STRAIN, RHIZOPUS ORYZAE NAF-032

本文运用单因素试验设计及正交试验设计理论确定高产Ｌ－乳酸突变菌株ＮＡＦ－０３２的最优种子培养基及培养条件。最优种子培养基（ｇ／１）：葡萄糖８０．０，氯化铵２．０，ＮａＨ２ＰＯ４ ０．３，ＫＨ２ＰＯ４ ０．３ＭｇＳＯ４·７Ｈ２Ｏ ０．２５，ＺｎＳＯ４·７Ｈ２Ｏ ０．０５。最优种子培养条件：温度３４℃，转速 ２００ｒ／ｍｉｎ，装液量６０ｍｌ（２５０ｍｌ三角瓶），一次性添加ＣａＣＯ３１０．０ｇ／１用于调节ｐＨ值，种子培养时间２２ｈ。

The optimal preculture medium and culture conditions for L-lactic acid high-producing strain Rhizopus oryzae NaF-032 were developed by single factor and orthogonal design theory. From the shake-flask experiments, the optimal preculture medium(g/l) and culture conditions were: glucose 80.0, NH4 Cl 2.0, KH2P O4 0.3, NaH2PO4 0.3, MgSO4·7H2O 0.25, ZnSO4·7H2O 0.05;34,200r/min, loading 60 ml in 250ml flask, 10.0g/L CaCO3 was added to control the pH of both, 22h culture age.