抗精子抗体和精子活力及顶体蛋白酶关系的研究

STUDY ON THE CORRELATION OF SPERM ANTIBODIES WITH SPERM MOTILITY AND ACROSIN ACTIVITY

本文研究了抗精子抗体与精子活力和顶体蛋白酶活性的关系。用凝集试验(GAT)和制动试验(SIT)检测男性不育患者血清中的抗精子抗体。同时用多次曝光法和底物膜法测定精子的前向运动速度和顶体蛋白酶活性。结果,当抗体滴度≥1:16时,可明显地降低精子的前向运动速度,且在制动试验阳性的患者降低更为明显,更精确而有力地证明了抗精子抗体对精子运动的抑制作用。抗精子抗体与顶体蛋白酶活性则无明显关系。

Correlation of sperm antibodies with sperm motility and acrosin activity was studied. Sperm antibodies of infertile men were examined by Gelatin Agglutination Test(GAT) and Sperm Immobilization Test(SIT). At the same time, sperm motility was examined by multiple exposure photography method and acrosin activity by substrate film technique. The results showed that, antibodies (especially found by using SIT)could significantly lower the average speed of sperm movement when titers was 1: 16 or higher. So our data exactly and strongly showed that sperm antibodies could inhibit sperm motility. Acrosin activity showed no correlation with sperm antibodies.