人类精子运动的浊度分析

TURBIDIMETRIC ANALYSIS OF HUMAN SPERM MOTILITY

浊度分析法是以位于比色杯底的细胞悬液里的精子首先会向上游入介质为基础的。由于这些精子不断地进入介质,就会产生一个随时间迁移而增加的吸光度值的变化。一般扫描持续600秒钟,可得到快速运动相精子(FRM)的百分含量和它们的平均速度(VRM)。用这种方法我们检测了50位有生育力的和23位患精子乏力型不育症的人精液。结果发现:正常人及乏力型不育患者FRM分别为4.15％和4％。YRM分别是79.11μm/sec和31.66μm/sec。而且无论是有生育力还是不育患者。FRM和YRM之间无相关性。我们应用浊度法所测得的快速运动相精子,很可能是完成受精的最好候选者。为此,这种方法可用作估价人类精子受精能力的指标。

A turbidimetric method is based on the fact that sperm so endowed will be the first to swin upward into medium from a concentrated sperm suspension at the bottom of an optical cuvette. This results in a time-depedent increase in turbidity in the medium which is recorded spectrophotometrically as an increase in absorbance. The determination requires 600 seconds and yields both the fraction of rapidly moving sperm (FRM) and their average velocity (VRM). Examination of 50 fertile human and 23 asthenozoospermia semen samples yielded FRM values of 4.15%, 4%, respectively, whereas values for VRM average about 79.11μm/ sec, 31.66μm/sec, respectively. There is no correlativity between FRM and VRM in fertile and infertile human. These vigorously motile sperms may be the best candidates for fertilization. It is suggested that this sample turbidimetric test can be used in evaluation of human semen as a possible indicator of fertilizing capacity.