摘要
局部枯斑法测定结果表明,番茄GCR—267品系叶片内ToMV的含量仅为番茄GCR-26品系叶片内病毒含量的1/40—1/50;GCR-267品系含有的Tm-2^(?)基因对ToMV增殖的抑制作用不受温度变化的影响;而ToMV在GCR-26体内的增殖却依赖于环境温度,高温对它有部分的抑制作用。用非变性聚丙烯酰胺凝胶电泳和SDS—聚丙烯酰胺凝胶电泳分析上述两个品系在常温和高温下接种ToMV后其叶片可溶性蛋白的变化,结果发现在GCR-26品系中,ToMV的增殖与寄主体内新产生的14.2KD蛋白呈正相关,而在GCR—267品系中未检测到这种蛋白。我们推测14.2KD蛋白可能是一种能参与或促进ToMV增殖过程的温度敏感因子,称之为番茄“S”蛋白。
ToMV concentrations in virus inoculated tomato plants were analyzed by local lesion test. The results showed that virus concentrations in resistan-t line GCR-267 was 1/40—1/50 that in susceptible line GCR-26. ToMV multiplication was strongly inhibited by gene Tm-2~a, which was not influenced by temperature. ToMV multiplication in GCR-26 was dependent on temperature, the higher the temperatature, the less the virus concentration. The changes of the soluble leaf proteins of tomato plants grown at constant or high temperature after inoculation with ToMV were analyzed by PAGE and SDS-PAGE. It was found that there was a positive relationship between ToMV multiplicaton and the production of new protein (MW. 14.2 KD) in GCR-26. This protein was not detected in GCR-267. It is suggested that 14.2 KD protein be a high temperature sensitive protein factor. We suggested it tomato'S'protein.
出处
《病毒学杂志》
CSCD
1989年第4期415-426,共12页
