胚胎分割,分割胚的体外发育和移植

Bisection of Embryos, Cultur of Demiembryos in vitro at l Fransfer

用玻璃微针徒手切割小鼠早期胚胎,分割的裸半胚在体外培养条件下对其体外发育率,发育阶段及胚泡大小进行观察。本实验徒手切割小鼠2细胞和8细胞阶段胚胎342枚,切割后成活的半胚为411枚,半胚成活率为60.08％(411/682)。2细胞和8细胞阶段切割的半胚成活率分别为86.3％(209/242)和45.7％(202/442),两者差异极显著(P<0.01),2细胞阶段切割成活率高于8细胞阶段。半胚体外培养24h,2细胞和8细胞切割的半胚发育率分别为78.14％(118/151)和90.14％(150/166)差异极显著(P<0.01),8细胞期半胚发育率明显高于2细胞期。半胚体外培养发育到胚泡期,由半胚发育成的胚泡其直径为80～100μm。与对照组相近。选择发育正常的胚泡,移植到假孕受体子宫,获得由半胚发育成的仔鼠8只。

Early embryos of micc. were biseeted with demiembryos were cultured in vitro for 24-48 developmental stage of demiembryos and size of bla with the nomal embryos which were not seeted. Ther ents. The youngs which developed from demiembry dred and fotrty two embryos were biseeted alt demiembryos were 411. Viable rate of demiembryos of 2 and 8 cell stage embryos biseeted were respeetively. The viable rate of 2 cell stage cmbryos After cultivation for 24h in vitro, the developmer cell and 8 cell stage were 78.14% (118/151) dle barchanded and biseeted activity, developmental rale, ere investgated in comparison yos were transffered to reeipi- tained evcntually. Three hun- the experiment. Survival of %(411 / 682). The viable rates 242) and 45.7%(202/442) was higher than 8 cell stage. liembryos obtained from 2 0/166) respectively. The developmental rate of 8 cell stage embryos were higher than 2 cell stage. Embryos were cul- tured in vitro to blastocyst stage and transferred to recipient mice. 8youngs were yielded from developed demiembryos.