Ki-67与Bcl-2在非霍奇金淋巴瘤中的表达及其临床意义

The expression and clinical significance of proliferative antigen Ki-67 and apoptosis-antagonizing antigen Bcl-2 in non-Hodgkin′s lymphoma

目的 探讨增殖相关抗原Ki 6 7和抗凋亡蛋白Bcl 2的检测 ,观察其在非霍奇金淋巴瘤(NHL)中的表达及临床意义。方法 用免疫组织化学染色的方法 ,对 35例NHL外科活检石蜡包埋组织切片进行Ki 6 7抗原和Bcl 2蛋白的检测。结果 (1)Ki 6 7在低度恶性NHL组中的表达低于高度恶性组 (P <0 .0 1) ,而Bcl 2在低度恶性NHL组中的表达高于高度恶性组 (P <0 .0 1)。 (2 )B细胞性NHL中Bcl 2的表达高于T细胞性NHL(P <0 .0 2 ) ,而Ki 6 7表达差异无显著性 (P >0 .1)。 (3)首次化疗缓解组中的Bcl 2表达低于未缓解组 (P <0 .0 1) ,而Ki 6 7表达差异无显著性 (P >0 .1)。 (4)高Ki 6 7表达组的生存期比低表达组短 (P <0 .0 5 ) ,而Bcl 2的表达在两组之间无差异。结论 增殖相关抗原Ki 6 7和抗凋亡蛋白Bcl 2的表达与NHL的恶性程度和预后密切相关 ,是了解肿瘤发生和判断NHL预后的有效指标。

Objective To evaluate the expression of proliferative antigen Ki 67 and apoptosis antagonizing protein Bcl 2 as well as their clinical significance in non Hodgkin′s lymphoma(NHL).Methods Ki 67 antigen and Bcl 2 protein were measured by immunohistochemistry on paraffin embedded slices in 35 cases of NHL.Results (1)The level of Ki 67 expression in low grade NHL was lower than that in high grade NHL( P <0.01),while Bcl 2 expression in lower grade NHL was higher than that in high grade( P <0.01).(2) The level of Bcl 2 expression in B cell NHL was higher than that in T cell NHL( P <0.02),but there was no difference in the expression of Ki 67.(3)The level of Bcl 2 expression in remission group was lower than that in non remission group after the first course of chemotherapy, but there was no significant difference in Ki 67 expression.(4)The survival time was longer in the group with Ki 67≤20% than that in the group with Ki 67 >20%, but there was no significant difference of survival time between the high and low expresion group of Bcl 2.Conclusions The level of Ki 67 and Bcl 2 expression was closely related with the grade of malignant and the prognosis of NHL. They were a pair of useful markers for understanding the generation and prognosis of NHL.