胃癌患者血清中NO、T-SOD及TNF-α的水平变化及临床意义

Significance of NO、SOD and TNF-αChanges in Serum from Gastric Cancer Patients

目的 探讨胃癌患者血清中一氧化氮 (NO)、总超氧化物歧化酶 (T -SOD)及肿瘤坏死因子 -α(TNF -α)的含量变化。方法 用生物化学方法测定胃癌患者血清NO、T -SOD含量 ;用EILSA法测定胃癌患者血清TNF -α含量 ,并进行统计学分析。结果 胃癌患者血清NO及T -SOD含量均显著低于正常对照组 (P值均 <0 .0 5 ) ,而血清TNF -α含量显著高于正常对照组 (P <0 .0 5 )。本实验还对胃癌患者中的 10例进行了肿瘤根治术前及肿瘤根治术后 10天左右标本的对比检测。结果表明 ,肿瘤根治术后该病患者血清NO及T -SOD含量较术前明显升高 (P值均 <0 .0 5 ) ,但仍低于正常对照组 (P值均 <0 .0 5 ) ,而血清TNF -α含量较术前明显降低(P <0 .0 5 ) ,但仍高于正常对照组 (P <0 .0 5 )。结论 胃癌患者血清NO及T -SOD含量降低 ;TNF -α含量增高 ,多项指标的联合检测对其发病机理探讨 ,疗效观察和预后判断均有一定临床意义。

Objectives To study the contents of the serum nitrie oxide (NO),superoxide dismutase (SOD) and turmor necrosis factor (TNF-α) of patients with gastric cancer patients. Methods The serum NO and SOD in gastric cancer patients were detected using biochemical methods;the content of the serum TNF-α was detected using ELISA methods and analyzed statistically. Results The serum NO and SOD contents were all lower than that of controls (P<0.05),the content of the serum TNF-α was significantly higher than that of controls (P<0.05). That removal of the carcinoma, the serum NO and SOD contents were significantly higher than that of the patients before operation (P<0.05), but still lower than that of controls (P<0.05), meanwhile, the serum TNF-α content was significantly lower than that of patients before operation (P<0.05), but still higher than that of controls (P< 0.05). Conclusion The serum NO and SOD contents in gastric cancer patients were lower, TNF-α conten was higher, the combination detections of the parmeters contribute significantly to studying the etiopathogenesis and therapeutic efficacy observations and prognosis judgements in clinics.