富硒大蒜对体内外人胃癌细胞生长的影响

In vivo and in vitro effects of selenium-enriched garlic on growth of human gastric carcinoma cells

目的 比较富硒大蒜、普通大蒜、亚硒酸钠以及普通大蒜与亚硒酸钠混合 (蒜硒联合 )处理影响人胃癌细胞生长的能力。方法 利用细胞计数、流式细胞术、Westernblot和裸鼠瘤体积测定等方法 ,观察富硒大蒜水溶物对离体培养的MGC80 3胃癌细胞系及其在裸鼠皮下生长的影响。结果(1)在离体条件下 ,富硒大蒜对MGC80 3细胞增殖有明显抑制作用 ,与等蒜量普通大蒜作用强度相似 ;等硒量亚硒酸钠抑制作用最弱 ,蒜硒联合抑制作用最强。 (2 )富硒大蒜、普通大蒜和亚硒酸钠处理2 4h后 ,未同步化的细胞G1期增多 ,已同步化的细胞S期增多 ;蒜硒联合处理则使未同步化和已同步化细胞G2 +M期增多。 (3) 4种处理 2 4h后 ,同步化细胞的Cdk2 CyclinE和Cdk4 CyclinD1复合物蛋白含量均降低。 (4)饲喂Balb/C裸小鼠含 1.6 7%富硒大蒜粉 (含硒 2 μg/g)的饲料 ,对移植瘤生长的抑制率达 2 9.92 % ;0 .83 %富硒大蒜、1.6 7%普通大蒜和 4.38μg/g亚硒酸钠 (含硒 2 μg/g)处理组未见明显抑制作用。 (5 ) 0 .83%富硒大蒜处理可诱发裸鼠单核细胞包裹肿瘤。结论 富硒大蒜能够抑制MGC80 3细胞在体外的生长 ,主要作用在于蒜。富硒大蒜对裸鼠移植胃癌有抑制作用 ,作用比普通大蒜和亚硒酸钠强。

Objective To compare the effects of selenium enriched garlic (Se garlic), garlic, Na 2SeO 3, and garlic+Na 2SeO 3 on growth of human gastric carcinoma cells.Methods In vivo and in vitro assays were carried out in the cultured human gastric carcinoma cell line MGC803 transplanted in the nude mice by cell count, flow cytometry, Western blot and neoplasm volume mesurement.Results The inhibition effect of garlic was similar to that of Se garlic, but Na 2SeO 3 was weaker than Se garlic. Combination of garlic and Na 2SeO 3 was stronger than Se garlic. In flow cytometry assay, the proportion of G 1 phase was increased after 24 hour treatment of Se garlic,garlic and Na 2SeO 3 in the non synchronized cells. However, the proportion of S phase was increased in the synchronized cells. The proportion of G 2+M phase was increased both in non synchronized and synchronized cells on treatment with the combination of garlic and Na 2SeO 3. The amount of Cdk 2 CyclinE complex and Cdk 4 CyclinD 1 complex was decreased in all treated synchronized cells in immunoprecipitation and immunoblot assays. The growth of MGC803 tumor in male Balb/c nude mice was inhibited by administration of 1.67% of Se garlic (Se 2 μg/g) in diet with an inhibition rate of 29.92% in tumor weight. Treatment of either 0.83% of Se garlic or 1.67% of garlic or 4.38 μg/g of Na 2SeO 3 (Se 2 μg/g) could not appreciably inhibit the growth of tumor. Wrapping of a number of monocytes around the tumor was induced in 62.50% of tumors in 0.83% of the Se garlic group.Conclusion In vitro, Se garlic is able to inhibit the growth of MGC803 cell through action of garlic. In vivo, Se garlic is able to inhibit growth of MGC803 tumor in nude mice by being better than garlic and selenite. [