RP-HPLC测定普藏红胶囊中阿魏酸的含量

STUDY ON DETERMINATION OF FERULIC ACID IN PUZANGHONG CAPSULES BY RP-HPLC

目的 :建立普藏红胶囊中阿魏酸含量的RP HPLC测定法。方法 :胶囊内容物先用 2 %碳酸钠水溶液提取 ,乙醚脱脂 ,然后用盐酸调pH1,再用乙醚提取出阿魏酸。在 μBondapakODS柱上分析 ,流动相为甲醇 水 (2 0∶80 ,磷酸调pH3.0 ) ,波长 30 0nm处检测 .结果 :阿魏酸在 10～ 5 0 μg·ml-1范围内 ,峰面积与浓度成良好线性关系 (r =0 .9998) ,加样回收率为 99.6 9% ,重复进样RSD为 0 .87% (n =5 ) ,重复性试验RSD为 1.14% (n =6 )。结论 :该法简便 ,快速 ,准确 。

OBJECTIVE:To develop a method to determine Ferulic acid in Puzanghong capsules by RP HPLC. METHODS:The ferulic acid in Puzanghong capsule was extracted with 2% Na 2CO 3 solution and removed ester component with ether.Then adjusted pH1 with hydrochloric acid and extracted with ether. It was analyzed on a μ Bondapak ODS column with methol water(20∶80 adjusted to pH3.0 with phosphoric acid).The detective wavelength was 300 nm.RESULTS:The calibration curves were linear( r =0.9998) within the range of 10～50 μg·ml -1 for ferulic acid and the average recovery was 99.69 % at three levels. The instrumental precision RSD was 0.87% (n =5) and repeatability test precision RSD was 1.14 %( n =6).CONCLUSION:The method is simple and quick with high accuracy, and it is suitable for the assay of the ferulic acid for Chinese angelica and compound preparation.