5-Br-DMPAP直接光度法测定血清铜

Methodology Research of Direct Assay For Cu in Serum With 5-Br-DMPAP

目的 :建立一种快速、简便、灵敏分光光度法测定血清铜 ;方法 :在表面活性剂 Triton X-1 0 0存在下 ,用 2 - ( 5 -溴 - 2 -吡啶偶氮 ) - 5 -二甲氨基酚 (简称 5 - Br- DMPAP)作显色剂直接光度法测定血清铜 ;结果 :该方法测定血清铜 ,显色络合物最大吸收波长为 5 60 nm,线性范围达 63.0 μ mol· L-1,表观摩尔吸光数为 5 .89× 1 0 4 L·mol-1·cm-1。回收率为 98.8%～1 0 1 .5 % ,变异系数 ( CV) 2 .3% ,与原子吸收分光光度法比较相关良好 ,Y=0 .95 5 X+ 0 .833,r=0 .9663,P>0 .0 5 ,36例健康人血清铜含量为 1 0 .0～ 2 3.6μmol· L-1( x± 2 s) ;结论 :该法血清用量少 ,不必去蛋白 ,具有操作快速、简便、结果灵敏可靠等优点 。

Objective:To establish a simple sensitive and stable method of determination of copper in serum by UV-Spectrophotometry.Methods:For determination of serum copper with 5-Br-DMPAP in the presence of surfactant Triton X-100.Results:The maximum absorption of the complex compound was at 560 nm,The linearity of test was up to 63 0 μmol·L -1 ,The molar absorption coefficient is 5.89×10 4 L·mol -1 ·cm -1 .The recoveries ranged from 98 8% to 101 5%.The CV was 2.3%.Comparing this method (Y)with AAS(X),we obtained the regression equation.Y=0.955X+0.833,r=0.9663,P>0.05,The reference interval (x±2s) was (10.0～23.6) μmol·L -1 (n=36).Conclusions:This method is simple.sensitive,rapid and accurate,and suitable for clinical application