用PCR方法检测粪便难辨梭状芽胞杆菌

Use of the Polymerase Chain Reaction for the Detection of Clostridium Difficile in Human Feces

目的 :采用 PCR方法检测粪便难辨梭状芽胞杆菌。方法 :北京协和医院的 36例应用抗生素后腹泻的病人粪便标本 ,采用 PCR方法对难辨梭状芽胞杆菌 (以下简称 C.D)的毒素 B基因和毒素 A基因进行扩增 ,同时进行厌氧细菌培养。结果 :PCR法毒素 B基因的阳性率为 46 .7% (16 /36 ) ,毒素 A基因为 36 .1% (13/36 ) ;用厌氧菌培养的阳性率为19.2 % (7/36 )。PCR检测 C.D毒素基因方法与厌氧培养法的一致性为 10 0 %。结论 :用 PCR方法检测 C.D比厌氧培养法检出率明显增高 (P<0 .0 5 ) 。

Purpose: Use of the PCR for the detection of clostridium difficile(C.D) in human feces. Methods: 36 patients with typical clinical manifestation of C.D colitis from the Peking Union Medical College Hospital were chosen for this study. PCR was used for detection of toxin A & B of C.D in stool specimens, while the cultures for C.D were performed under anaerobic conditions. Results: 46.7%(16/30) stool specimens were positive for toxin B, 36.1%(13/36) were positive for toxin A, 19.2%(7/36) were positive for anaerobic culture. The consistency between the method of PCR and anaerobic culture was 100%. Conclusion: The method of PCR for detection of C.D in human feces is more sensitive than of the culture (P<0.05) and there is high consistency with the anaerobic culture.