黄参方剂诱导胃癌癌前病变细胞凋亡和影响bcl-2蛋白表达的研究

Research on the Effect of Huang Shen Preparation on the Induction of Apoptosis of Gastric Pre-carcinoma and the Expression of bcl 2 Protein

目的 :探讨黄参方剂对胃癌癌前病变细胞凋亡及相关基因表达的影响。方法 :将 72例胃癌癌前病变患者随机分为 2组 :治疗组 37例 ,口服黄参方剂 6 0 ml/ d;对照组 35例 ,口服维酶素片 12片 / d,疗程 2月。治疗后胃镜同一部位取材 ,检测治疗前后细胞凋亡指数和 bcl- 2蛋白的表达。采用原位末端标记法 (TUNEL )检测细胞凋亡情况 ,采用免疫组化法检测 bcl- 2蛋白的表达。结果 :黄参方剂组服药前后细胞凋亡指数分别为 19.8± 9.6 %和 32 .8± 16 .2 % ,服药后细胞凋亡指数显著高于服药前 (P<0 .0 5 )。 bcl- 2蛋白阳性率治疗前后分别为 5 2 .8%和 2 6 .4% ,治疗前后比较具有显著性差异 (P<0 .0 5 )。而对照组细胞凋亡指数和 bcl- 2蛋白阳性率在服药前后比较均无显著性差异 (P>0 .0 5 )。结论

Purpose: To evaluate the effects of Huang Shen preparation on apoptosis and expression of bcl 2 in gastric precancerous lesions. Methods: 72 cases of gastric precancerous lesions were divided into two groups at random-a treatment group of 37 cases and a control group of 35 cases. The treatment group was treated with Huang Shen preparation 60 ml a day for two months. The control group was treated with wei mei su 12 tablets a day for two months. TUNEL method was used to detect apoptosis. Immunohistochmical analysis was used to detect the expression of bcl 2 protein. Results: The treatment group changes as follow: Apoptosis index increased from 19.8±9.6% to 32.8±16.2%, the expression rate of bcl 2 decreased from 52.8% to 26.4%. The change of apoptosis indexes and expression rate of bcl 2 were statistically significant (P<0.05). In control group it did not change significantly(P>0.05). Conclusions: It is necessary to study the therapeutic value of Huang Shen preparation on gastric precancerous lesions, since it can induce apoptosis of gastric precancerous lesions cells.