肝癌抗原特异性的检测

Identification of specificity of hepatocellular carcinoma antigen

目的 检测两种新的人肝细胞癌 (HCC)抗原 (编号为HCC 1 8a和HCC 3 13)对HCC的特异性。方法 应用重组克隆表达抗原的血清学技术 (SEREX) ,用异体HCC血清和非HCC血清检测两种新的HCC抗原的特异性。结果 相应的抗体主要见于HCC患者 ,阳性反应率分别为 80 % (8/ 10 )及 90 % (9/ 10 ) ;胃癌为 0及 2 0 % (1/ 5 ) ;正常人各为 7.1% (2 / 2 8) ;乳癌为 0。结论 两种新的HCC抗原对HCC有高度特异性 ,可考虑作为HCC血清学诊断的指标之一。

Objective Identification of antigenic specificity of two new hepatocellular carcinoma (HCC) to HCC.Methods Serological identification of antigens by recombinant expression cloning technique (SEREX) was used in order to identify specificity of two HCC antigens in allogenic HCC sera and other sera including gastric carcinoma, breast cancer and normal individuals.Results The antibody positive responses to two HCC antigens were respectively 80%(8/10) and 90%(9/10) in HCC, 0 and 20%(1/5) in gastric carcinoma, both 7.1%(2/28) in normal individuals and zero in breast cancer.Conclusion Two new HCC antigens which could be considered as one of serological diagnostic in HCC showed stronger specificity in HCC cases.