局灶性脑缺血鼠血浆细胞因子变化及尼莫地平的治疗效果

CHANGES OF CYTOKINES IN RATS PLASMA AFTER FOCAL BRAIN ISCHEMIC REPERFUSION AND EFFECTS OF NIMODIPING

目的 探讨脑缺血再灌注后血浆TNFα、IL - 1β变化及尼莫地平对其含量的影响。 方法 采用线栓法制备大鼠局灶性脑缺血再灌注模型 ,用放射免疫方法检测脑缺血再灌注后血浆TNFα、IL - 1β变化 ,以及尼莫地平对TNFα、IL - 1β的拮抗作用。 结果 缺血再灌组与假手术组相比 ,血浆TNFα、IL - 1β含量明显增加 ,应用尼莫地平组TNFα、IL - 1β水平均降低。 结论 TNFα、IL - 1β参与了局灶性脑缺血再灌注损伤的病理过程 ,尼莫地平通过降低TNFα、IL - 1β的含量发挥一定的脑保护作用。

Objective Inflammatory cytokines and endothline play an important role in brain injury during ischemic reperfusion.In this study,inflammatory cytokines were investigated to determine the changes of TNFα and IL-1β after brain ischemic reperfusion and interferential effect of nimodiping on TNFαand IL-1β.Methods The animal models of the focal middle cerebral arterial ischemic reperfusion were established in Wistar rats.Nimodiping was administered by introvenous drip.The plasma levels of TNFα、IL-1βwere measured by radioimmunoassay.Rusults The levels of TNFαand IL-1βin plasma were elevated after 1h brain ischemia and 1h reperfusion in rats.Nimodiping can significantly reduce the content of TNFαand IL-1β in plasma.Conclusion The results indicate that TNFαand IL-1β participate in cerebral ischemia reperfusion lesion,which can be reduced by nimodiping,and nimodiping can protect nerve cells from injurg through inhibiting TNFαand IL-1β production.