子宫内膜癌P^(53)基因表达与细胞增殖活性的研究

Studies on the relation of P^(53) gene expression of endometrial cancer and activity of cell malignant proliferation

目的 探讨 P5 3基因表达在子宫内膜癌发生、发展中的作用。方法 用原位杂交法、免疫组化法及苏木素 -HE染色 ,分别测定 37例子宫内膜癌病人癌组织中 P5 3m RNA的表达、 PCNA指数 (PI)及细胞分裂指数 (MI) ,并与正常子宫内膜及增生性子宫内膜相比较。结果 子宫内膜癌组织中 P5 3与 PI、MI均明显高于正常及增生性子宫内膜组 ,且三者的数值均随疾病的进展而升高 ,差别均有显著性意义 (P <0 .0 5 ) ,并且升高的趋势呈正相关关系。结论 P5 3基因的突变或异常表达与子宫内膜癌的发生、发展有密切关系 ,P5 3基因的异常表达常与更具侵袭性行为的生物学行为有关。 P5

Objective\ To investigate the role of expression of P 53 gene in development and progress of endometrial cancer Method\ Useing hybridization in situ,immunohistochemical technique and HE staining,we detected the expression of P 53 mRNA,PCNA index(PI) and cell mitosis index(MI), respectively Results\ The value of P 53 and PI,MI increased with progress of the disease The trend was basically positive correlation Conclusion\ The mutation or abnormal expression of P 53 gene is closely related to development and progress of the endometrial cancer Abnormal expression of P 53 gene is concern with more invasive biological behavior The detection of P 53 gene and cell proliferation activity is valuable for judging prognosis of endometrial cancer \ \