黄芪痰瘀饮对大鼠高脂血症并急性心肌缺血的影响

Experimental Study on Effects of HQTYY on Rats with Hyperlipemia and A-cute Myocardial Ischemia

目的黄芪痰瘀饮是本院临床治疗气虚血瘀、痰浊内阻型冠心病的常用自拟方,本研究利用大鼠实验模型,进一步观察了药物对高脂血症并急性心肌缺血的作用。方法实验分4组,模型组大鼠灌饲脂肪乳剂1ml/100g腹腔注射垂体后叶素Pit0.7u/100g药物组同时灌饲黄芪痰瘀饮1ml/100g正常对照组及高脂对照组予等量生理盐水对应给药,每日1次,第8d观测心电图变化、血清生化指标及心肌结构变化。结果高脂对照组与正常对照组相比血清甘油三酯TG、低密度脂蛋白LDL明显升高,超氧化物歧化酶SOD含量明显降低P<0.01～0.001;高脂加Pit组与高脂对照组相比TG、LDL进一步增高,SOD含量进一步下降,高密度脂蛋白HDL亦明显下降,过氧化脂质LPO、肌酸激酶同功酶MBCK-MB、ST段显著升高P<0.05～0.001;黄芪痰瘀饮组与高脂加Pit组相比TG、LDL、CK-MB较低,HDL较高P<0.05～0.001,而SOD、LPO、ST段变化与单纯高脂对照组相比无显著性差异P>0.05,光镜下见黄芪痰瘀饮组心肌受损程度轻于高脂加Pit组。结论Pit致急性心肌缺血可以恶化高脂血症,并使机体内氧自由基生成增多;黄芪痰瘀饮具有调脂、对抗氧自由基、抗Pit致急性心肌缺血性损伤的作用。

HQTYY,a compound prescription wich mainly consisted with Radix As-tragali,Radix Salviae Miltiorrhizae,Fructus Trichosanthis and Bulbus Allii Macrostemi,is often used to treat coronary heart diseases in clinic.The present study was undertaken to investigate the effects of HQTYY on hyperlipemia and pituitrin induced acute myocardial ischemia in rats.Methods:The male Wistar rast were di-vided into four groups.They were perfusated with adipose emulsion (1 ml/100g)or/and injected with pituitrin (0.7u/100g) in intraperitoneal one time a day respec-tively.The HQTYY(1ml/100g)was administered in the treatment group in the mean time.At the eighth day,the ECG,some serum parameters and the variances of my-ocardial structure were detected and comparted.Results:The TG,LDL Contents in hyperlipemia control group were higher than those in normal control group,whereas the SOD was decreased significanly (P<0.01～0.001).The parameters above mentioned were aggravated when pituitrin was used.In addition,the HDL decreased and LPO.CK-MB and ST segment increased in this group (P<0.05～0.001).In contrast to this group,the TG,LDL,CK-MB were relieved and the HDL was a rel-atively high when administraten HQTYY(P<0.05～0.001).And the contents of SOD and LPO and the ST segment were similar as the hyperlipemia control group (P<0.05).Besides,the present study indicated that the injury of myocardial cells was alleviated in HOTYY group identified by microscope.Conclusion:The acute my-ocardial ischemia induced by pituitrin can deteriorate the hyperlipemia and can in-crease the production of the oxygen free radicals in the body.The HQTYY can reg-ulate the lipid metabolism,scavenge the oxygen free radicals and protect the my-ocardium from the acute ischemic injury caused by pituitrin.