参麦注射液对兔肠缺血再灌注后肺的保护作用

The protective effect of Shen Mai Injection on lung after intestinal ischemia reperfusion in rabbits

目的 :研究参麦注射液对兔肠缺血再灌注后肺的保护作用。方法 :利用肠系膜上动脉夹闭方法 ,制备肠缺血再灌注模型 ,将家兔随机分为正常对照组、缺血再灌组、参麦治疗组 ,分别测定不同时段血浆肿瘤坏死因子 (TNF)活性 ,并于再灌4h后处死动物 ,开胸取肺 ,测肺组织匀浆TNF活性 ,并做肺组织病理检查。结果 :缺血再灌组血浆TNF活性 ,缺血再灌后与缺血前比较 ,肠缺血1h就显著升高 (P<0.01),再灌1h继续升高 (P<0.01) ,以后逐渐下降 ;参麦治疗组与缺血再灌组同时刻比较 ,再灌1h、2h皆有显著性差异(P<0.05)。肺组织匀浆TNF活性 ,缺血再灌组与正常对照组比较有显著性差异(P<0.05) ,而参麦治疗组与正常对照组、缺血再灌组比较无显著性差异。肺组织病理 ,缺血再灌组与正常对照组比较有急性肺损伤的改变 ,参麦治疗组与正常对照组比较变化不明显。结论

Objective: To study the protective effect of Shen Mai injection on lung after intestinal ischemia reperfusion of rabbits. Methods: Rabbits were divided into three groups: normal control group,inchemia reperfusion group and Shen Mai treatment group. Plasma TNF activity in different time points was observed. The TNF activity and pathologic changes in lung were observed.Results: Plasma TNF activity was enhanced at 1st post ischemia (P<0.01). High level of TNF was at 1st after reperfusion (P<0.01) but was lower then that of in inchemia reperfusion group. Shen Mai treatment group was obviously lower (P<0.05) at 1st and 2nd points than inchemia reperfusion. The TNF activity in lung was obviously higher in ischemia reperfusion group than that of normal control group. But Shen Mai treatment group was not obviously changed than normal group. Lung pathological examination showed that actue lung injury was more predominant in ischemia reperfusion group than that of normal control group but lung pathological examination was not obviously changs in Shen Mai treatment group than normal ctroul group. Conclusion: The early treatment with ShenMai injection can protect lung after intetinal ischemia reperfusion.