银杏外种皮多糖抑制小鼠肝癌及诱导肝癌细胞凋亡的研究

Studies of Ginkgo Biloba Testa Polysaccharides in Inhibiting Liver Cancer and Inducing Apoptosis of the Liver Cancer Cell in Mice

目的：研究银杏外种皮多糖（Ｇｉｎｋｇｏ ｂｉｌｏｂａ ｅｎｏｃａｒｐ ｐｏｌｙｓａｃｃｈａｒｉｄｅｓ，ＧＢＥＰ）对小鼠肝癌的抑制作用及作用机制。方法：建立小鼠肝癌（Ｈｅｐｓ）实体瘤及腹水癌模型，观察抑瘤率和小鼠的生命延长率，并用流式细胞术（ＦＣＭ）检测小鼠肝病实体瘤的细胞周期及癌细胞凋亡率。结果：ＧＢＥＰ在５０，１００和 ２００ｍｇ·ｋｇ～（－１）剂量下，对小鼠肝癌的抑瘤率皆大于３０％，能延长荷腹水型肝癌小鼠的生存期；随ＧＢＥＰ剂量增大，Ｇ０－Ｇ１期和Ｓ期细胞（％）不断减少，而Ｇ２—Ｍ期细胞及凋亡细胞（％）则不断增加。结论：ＧＢＥＰ可抑制小鼠肝癌（Ｈｅｐｓ）．其作用机制可能与阻止Ｇ２—Ｍ期细胞转换而影响癌细胞在细胞周期中的进程和干扰Ｓ期细胞ＤＮＡ合成以及诱导小鼠肝癌细胞凋亡有关。

Objective: To study the anticancer action of Ginkgo Biloba testa polysaccharides (GBTP) on mice liver cancer and its mechanism. Method: Mouse models of the solid tumor of liver cancer and ascitic liver cancer were established. The inhibitory rate of solid tumor and the life prolongation rate of ascitic cancer were observed. The cell cycle and apoptosis of the solid tumor cells were measured by Flow Cytometry (FCM). Results: The inhibition rate of GBTP (50, 100 and 200 mg. kg ^(-1) . d^(-1)) on the solid tumor was over 30% and the life of ascitic cancer bearing mice was also prolonged. GBTP could decrease the number of cells in G0 - G1 and S phase and increase the number of cells in G2 - M phase and the number of apoptotic cells in a dose - effect manner. Conclusion: GBTP could suppress liver cancer in mice. The anticancer mechanism was probably associated with the arrestment of cell cycle from G2 - M phase to G1 phase transition, interference of DNA synthesis in S phase, and the induction of cancer cell apoptosis.