摘要
用薄层色谱法对巴戟补肾液中的巴戟、熟地、淫羊藿、人参、牛膝、山药进行了定性鉴别。用ＨＰＬＣ法对制剂中的淫羊藿苷进行了含量测定。流动相：甲醇－０．５％ＨＣｌＯ４（５５：４５），流速：０．８ｍＬ／ｍｉｎ；柱温：４０℃；检测波长：２７０ｎｍ；平均回收率为９７．３６％，ＲＳＤ＝２．６１％（Ｎ＝５），为该制剂的质量评价提供了科学依据。
The identification of Radix Morindae Officinalis, Radix Rehmanniae Preparata, Herba Epimedii, Radix Ginseng, Radix Achyranthis Bidentatae, Rhizoma Dioscoreae in Baji Bushen decoction was carried out respectively by TLC. The content of icariin in the preparation was determined by HPLC. Chromatocolumn was adopted, methanol - 0. 5 % HClO4 (55: 45) as the mobile phase, flow - rate at 0.8 mL/min, column temperature at 40℃, detection wavelength at 270nm, the mean recovery is 97. 36%and RSD is 2.61% (N=5). It is indicated that the methods can be used for the quality control of Baji Bushen decoction.
出处
《中药新药与临床药理》
CAS
CSCD
2001年第5期362-365,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
