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螺旋藻胞外和胞内核酸酶活性研究 被引量：6

Research on Extracellular and Intracellular Nuclease Activity of Spirulina
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摘要 通过分析比较 2个螺旋藻物种 7个品系胞外和胞内核酸酶活性 ,确定了抑制胞外和胞内核酸酶活性的方法。用液体培养基清洗两次可消除胞外核酸酶活性 ,添加 EDTA(2 m mol L-1)可显著地抑制胞内核酸酶活性。高温 (6 5℃ ) Seven axenic strains of Spirulina were analysed for the extracellular and intracellular nuclease activity. Methods for inhibiting the extracellular and intracellular nuclease activity were developed. The activity of extracellular nuclease could be removed by washing the cells with Zarrouk medium twice. 2 mol L 1EDTA in Zarrouk medium and treatment at 65℃ could also inhibit the intracellular nuclease activity significantly.
作者 王高歌 杨官品 茅云翔 张宝红 张学成
机构地区 青岛海洋大学海洋生命学院
出处 《青岛海洋大学学报（自然科学版）》 CSCD 北大核心 2001年第6期883-888,共6页 Journal of Ocean University of Qingdao
基金 国家高技术研究发展计划项目 ( 81 9-0 6 -1 0 )资助
关键词 螺旋藻 胞外核酸酶活性 胞内核酸酶活性 EDTA Spirulina activity of extracellular nuclease activity of intracellular nuclease
分类号 Q949.221 [生物学—植物学]
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  • 1孙军,金苹,徐旭东,施定基,李令媛,茹炳根.小鼠金属硫蛋白──I　cDNA在蓝藻中的克隆和表达[J].生物工程进展,1994,14(6):39-42. 被引量：15
  • 2张智清,姚立红,侯云德.含P_RP_L启动子的原核高效表达载体的组建及其应用[J].病毒学报,1990,6(2):111-116. 被引量：178
  • 36，Lee D W, et al. Characterization of Synechocystis PCC6803 transformed w ith cryIVD gene of Bacillus thruingiensis subsp. Morrisoni PG-14 and its mo squitocidal effect on Anopheles sinesis. [J] Korean J Appl Entomol, 1996, 35:66～73
  • 47，Wu X, et al. Mosquito larvicidal activity of transgenic Anabaena strain PCC7120 expressing combinations of genes from Bacillus thruingiensis subsp. [J] Israelensis. Appl Environ Microbiol, 1997, 63:4971～4974
  • 58，Sangthongpitag K, et al. Cloning and expression of the Bacillus sphaericu s 2362 mosquitocidal genes in a non-toxic unicellular cyanobacterium, Synec hococcus PCC6301.[J] Appl Microbiol Biotechnol, 1997, 47:379～384
  • 69，Kawata Y, et al. Expression of salmon growth hormone in the cyanobacterium Agmenellum quadruplicatum.[J] Biotechnol Lett, 1991, 13(12):851～856
  • 710，Takeyama H, et al. Expression of the eicosapentaenoic acid synthesis cluster from Shewanella sp. in a transgenic marine cyanobacterium. Synech ococcus sp. [J] Microbiol 143(Pt 8): 2725～2731
  • 811，Sakai M, et al. Photosynthetic conversion of carbon dioxide to ethylene b y the recombinant cyanobacterium, Synechococcus sp. PCC7942, which harbors a gene for the ethylene forming enzyme of Pseudomonas syringae. [J] J Ferme nt Bioeng, 1997, 84:434～443
  • 912，Mermet-Bouvier P, Chauvat F. A conditional expression vector for the cyan obacteria Synechocystis sp. strains PCC6803 and PCC6714 or Synechococcus sp. strains PCC7942 and PCC6301. [J] Curr Microbiol, 1994, 28:145～148
  • 1013，Harker M, Hirschberg J. Biosynthesis of ketocarotenoids in transgenic cyan obacteria expressing the algal gene for β-C-4-oxygenase, crtO. [J] FEB S Letters, 1997, 404: 129～134

共引文献199

同被引文献33

  • 1茅云翔,王高歌,张宝红,杨官品,隋正红,张学成.建立螺旋藻转基因体系初报[J].中国海洋大学学报（自然科学版）,2000,33(z1). 被引量：9
  • 2甘旭华,唐欣昀,刘广金,史成颖,汪本凡.螺旋藻的纯化[J].微生物学通报,2005,32(2):1-4. 被引量：13
  • 3秦松,王希华,童顺,曾呈奎.钝顶螺旋藻部分原生质体及单细胞的制备与培养[J].海洋与湖沼,1995,26(1):109-112. 被引量：24
  • 4曹媛媛,甘旭华,赵良侠,夏觅真,刘广金,唐欣昀.紫外线和^(60)Coγ射线对钝顶节旋藻(Arthrospira platensis)的诱变效应[J].激光生物学报,2006,15(5):478-482. 被引量：7
  • 5Gan X,Tang X, Shi C, Betal, et al. Preparation and regeneration of spheroplasts from Arthrospira platensis (Spirulina) [J]. J of Appl Phycology, 2004, 16(6) : 513 -517.
  • 6Vonshak A. Spirulina platensis ( Anthrosprira ) : physiology, cell-biology and biotechnology [ A ]. Taylor and Francis, USA, 1997,205 - 212.
  • 7Tragut V, et al. Characterization of DNA restriction-modification systems in Spiμlina platensis strain pacifiea [J]. Journal of Applied Phycology, 1995,7 (6) :561 - 564.
  • 8[4]Lanfaloni L,Grifantini R,et al.Production and regeneration of spheroplasts From the cyanobacterium SpirμLina platensis.FEMS Microbiology Letters,1989,59:141 ～146
  • 9[6]Tragut V etal.Characterization of DNA restriction-modification systems in SpirμLina platensis strain pacifica.Journal of Applied Phycology,19957(6):561～564
  • 10[11]萨姆布鲁克J(美),弗里奇EF,曼尼阿蒂斯T.分子克隆实验指南(第二版)[M].北京:科学出版社,1999

引证文献6

二级引证文献7

青岛海洋大学学报（自然科学版）
2001年 第6期

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