突变型p53、PCNA表达和幽门螺杆菌感染与胃黏膜病变

Expressions of mutant p53,PCNA and Hp infection in the diseases of gastric mucosa

目的 研究突变型 p5 3、增殖细胞核抗原 (PCNA)和幽门螺杆菌 (Hp)感染与胃黏膜良、恶性病变之间的关系。 方法 采用免疫组织化学S -P法 ,对慢性浅表性胃炎 (CSG )、慢性萎缩性胃炎(CAG)、不典型增生 (GED)及胃癌 (GCA)共 115例胃镜活检标本进行突变型 p5 3、PCNA和幽门螺杆菌感染进行检测。 结果 突变型p5 3在CSG及CAG表达均为阴性 ,在GED表达阳性 2 4% ,在GCA表达阳性 5 5 % ,差异有显著性 (P <0 .0 1)。PCNA反映细胞增殖活性 ,在CSG、CAG、GED、GCA的表达有显著递增性 (P <0 .0 5 ) ;幽门螺杆菌感染在CSG检出率为 36 %、CAG为 48% ,GED为 72、GCA为 80 % ,与 2 0例正常胃黏膜Hp检测率 2 0 %作对照 ,除了CSG外 ,其余几组均有显著性差异 (P <0 .0 5 )。Hp感染与PCNA表达强级 (阳性细胞 >5 0 % ) ,与 p5 3阳性病例均有显著相关性 (P <0 .0 1)。 结论 突变型 p5 3、PCNA及幽门螺杆菌感染在胃黏膜不典型增生、胃癌的发生、发展中有一定作用 ;

Objective To investigate the expressions of mutan p53,proliferating cell nuclear antigen (PCNA) and Hp infection in the diseases of gastric mucosa. Methods The expressions of mutant p53, PCNA and Hp infection were detected by S-P immunochistochemical method in 25 patients with chronic superficial gastritis (CSG),25 patient with chronic atrophic gastritis(CAG),25 patients with gastric epithelial dysplasia(GED) and 40 patients with gastric carcinoma (GCA). Results Positive expression rates of mutant p53 in CSG and CAG were zero,but the rates were 24.0%(625) in GED and 55%(22/40) in GCA.There were significant differences among these groups (P<0.01).Positive expressions of PCNA increased markedly and progressively in CSG,CAG,GED and GCA,there were significant differences among them(P<0.05).Positive rates of Hp infection in CSG,CAG,GED,GCA and normal gastric mucsa was 36%,48%,72%,80% and 20%,respectively(P<0.05). Hp infection was correlated with expression of PCNA and mutant p53. Conclusion Expressions of mutant p53,PCNA and Hp infection may be related with GED, genesis and development of GCA.