乙型肝炎患者血清中白细胞介素6、12水平的检测及其临床意义

Detection of interleukin-6 and -12 in hepatitis B patients and its clinical significance

目的 探讨白细胞介素６（ＩＬ－６）、白细胞介素１２（ＩＬ－１２）在乙型病毒性肝炎免疫发病机制中的意义。方法 利用双抗 体夹心－酶联免疫吸附实验（ＥＬＩＳＡ）检测慢性乙型肝炎、急性乙型肝炎、重型乙型肝炎患者及正常对照组血清中ＩＬ－６、 ＩＬ－１２水平。结果 慢性乙型肝炎组ＩＬ－６、ＩＬ－１２值分别为（１９９．７±２６．９）、（２４．６±１３．４）ｐｇ／ｍｌ；急性乙型肝炎组分别为 （１２９．５±２２．８）、（１３５．３±６０．８）ｐｇ／ｍｌ；重型乙型肝炎组分别为（２８６．１±５６．７）、（ｌ９．７±９．０）ｐｇ／ｍｌ；正常对照组分别为（５６．４± １２．９）、（３４．７±１１．８）ｐｇ／ｍ１。结论ＩＬ－６的变化与乙型肝炎肝细胞损害程度密切相关，而ＩＬ－１２则在机体的抗病毒免疫应 答中起重要作用．其值升高可能预示肝病向良好转归发展。

Objective To investigate the clinical significance of detecting interleukin-6 (IL-6) and interleukin-12 (IL- 12) in the immunological mechanism of hepatitis B virus infection (HBV). Methods Serum IL-6 and IL-12 levels were determined using enzyme-linked immunosorbent assay in patients with chronic, acute or advanced hepatitis B as well as in healthy subjects. Results In chronic, acute, severe hepatitis B patients, serum IL-6 levels were significantly elevated as hepatitis worsened (199.7±26.9, 129.5±22.8, 286.1 ±56.7 pg/ml respectively), compared with the normal control levels (56.41 ± 12.9 pg/ml). IL-12 levels, in contrast, tended to be lowered with the deterioration of hepatitis (24.6±13.4, 135.3±60.8, 19.7±9.0 pg/ml respectively m that order), with the control level of 34.7 ± 11.8 pg/ml. Conclusion. Serum IL-6 level is closely correlated to the degree of hepatocyte damage in hepatitis B, while IL-12 may be instrumental in the defense mechanism against HBV infection, and IL- 12 level elevation can be indicative of hepatitis recovery.