庚型肝炎病毒E2区的基因异质性(英文)

Genetic Heterogeneity in E2 Region of Hepatitis G Virus

目的 :检测庚型肝炎病毒E2区的基因异质性。方法 :通过半巢式逆转录多聚酶链反应检测HGV。对RT PCR阳性标本直接自动测序或者克隆后测序。结果 :通过比较不同时期HGVE2区的核苷酸变化 ,估计该区每个位点的年突变替换率为 3× 10 3 次。HGVE2区的基因异质性可直接自动测序发现并通过克隆后测序加以证实。该区的基因异质性程度在干扰素治疗后均有下降。结论 :基因异质性存在于HGV的E2区 ,干扰素治疗可减少基因异质性。

Objective:To detect the genetic heterogeneity in E2 region of Hepatitis G Virus(HGV).Methods:HGV RNA was detected by a semi nested RT PCR.The positive samples of RT PCR were sequenced directly or cloned.Results:By comparison of the nucleotide changes in three E2 sequences at different times,it was estimated that HGV had a mutation rate of 3×10 3 substitutions/site/year in E2.We observed genetic heterogeneity in E2 of HGV by direct sequencing of the semi nested RT PCR products and confirmed this by sequencing analysis of clones.The degrees of gene tic heterogeneity were decreased by IFN treatment in our patients.Conclusion:Genetic heterogeneity is existed in the E2 region of HGV and decreased by IFN therapy.The detected sequence in E2 is the poorly conserved region of HGV.