输入供体血对延长大鼠移植胰腺功能存活的初步研究

THE INFLUENCES OF DONOR BLOOD INJECTION UPON PROLONGATION OF PAN CREATIC ALLOGRAFT SURVIVAL IN RATS

目的 探索供体血输入同种大鼠对诱导胰腺移植耐受的可能性。方法 用雄性Wistar大鼠作为实验的供受体 ,胰腺移植当天取供体大鼠全血 1ml ,注入糖尿病受体大鼠腹腔 ,辅以短期的硫唑嘌呤 (Aza)。结果 移植胰腺功能存活时间为 2 8～ 112天 (平均 64 .2天 ) ,与单纯用硫唑嘌呤组 ( 10 .2天 )或单纯输入供体血液组 ( 9.8天 )相比 ,移植胰腺功能存活时间显著延长 (P<0 .0 1)。结论 移植前短期使用免疫抑制剂以及移植当天输入供体血液 ,能延长大鼠移植胰腺功能存活时间。

Objective To investigate the effe ct of donor blood transfusion on inducing pancreatic allograft tolerance in outbre d rat model. Methods Wistar male rats were used as blood an d pancreas donor, and diabetic recipients. One ml of donor blood injected into a bdomen of diabetic recipients on the day of transplantation and azathioprine gi ven 2 days pretransplant and continued for three days. Results Pancreas allograft survival was signifi cantly prolonged (28 to 112 days, media survival time 64.2 days). One ml of dono r blood alone injected into the abdomen and azathioprine given alone 2 days pret ransplant did not improve allograft survival (media survival time 9.8 vs 10.2 da ys). Conclusion Donor blood injected on the day of transplantation and a 3 days course of azathioprine started 2 days pretransplan t have strong synergism in inducing long term graft survival in this rat model.