香烟提取物的致突变性研究

Study on the Mutagenicity of Total Particle Material Extracted From Cigarette

[目的 ]实验观察香烟提取物 (TPM )的致突变性。 [方法 ]取香烟TPM样品 11个 ,按不同剂量 ( 10 0、5 0 0、10 0 0 μg/皿 ) ,采用平板掺入法进行Ames试验检测TPM的致突变性 ;按 30、10 0、30 0mg/kg剂量进行小鼠骨髓微核试验。 [结果 ]Ames试验样品在不经活化时 ( -S9) 5 0 0、10 0 0 μg /皿 ,TA98、TA10 0 、TA97、TA10 2 Rt/Rc值 >2有剂量 反应关系 (r =0 93,P <0 0 1) ;样品经活化后 ( +S9) 5 0 0、10 0 0 μg/皿 ,TA98、TA10 0 、TA97、TA10 2 Rt/Rc值均 >2。 2 5个剂量组微核阳性率与对照组比较差异有统计学意义 (P <0 0 5或P <0 0 1)。 [结论

Objective\]To observe the mutagenicity of total particle material (TPM) extracted from cigarette.\11 samples were taken, the mutagenicity of TPM with different doses (100,500,1 000 μg/plate) was tested by Ames test According to doses (30,100,300 mg/kg) micronucleus test in bone marrow of mice,to examine mutation rate of micronucleus.\Ames test without rat liver homogenate (-S 9) was 500,1 000 μg/plate TA 98 ,TA 100 ,TA 97 ,TA 102 Rt/Rc>2, which show a dose response relationship (r=0 93,P<0 01) Ames test with rat liver homogenate (+S 9) was 500,1 000 μg /plate TA 98 ,TA 100 ,TA 97 ,TA 102 Rt/Rc>2.Mutation rate of micronucleus in 25 doses group was significantly higher than that in control group (P<0 05,P<0 01).\[Conclusions\]TPM may has effect of mutagenicity.